THE REQUIREMENT OF DIPHOSPHOPYRIDINE NUCLEOTIDE FOR CHOLINE DEHYDROGENASE ACTIVITY

Diphosphopyridine Nucleotide - linked Aldehyde Dehydrogenase

In continuation of our investigation of liver diphosphopyridine nucleotide-linked aldehyde dehydrogenase (1)) we have undertaken a study of various classes of inhibitors of the enzyme. Interest in aldehyde oxidation has been increasing in recent years from several points of view. The classical problem of ethanol oxidation in viva still has many unresolved facets (Z), and the enzymes responsible...

Aldehyde dehydrogenase, a diphosphopyridine nucleotide-linked enzyme.

The dismutation of acetaldehyde to ethyl alcohol and acetic acid has been shown to occur in animal tissues (1, 2). The dependence of this reaction on diphosphopyridine nucleotide was demonstrated by von Euler and Brunius (3). Partial purification of the responsible enzyme system was achieved by Dixon and Lutwak-Mann (4). These authors clearly demonstrated that the mutase activity was independen...

Diphosphopyridine nucleotide isocitric dehydrogenase from animal tissues.

Glyceraldehyde phosphate dehydrogenase activity with triphosphopyridine nucleotide and with diphosphopyridine nucleotide.

The results presented here are part of a study of pentose synthesis in Alcaligenes fuecalis. The mechanism of pentose synthesis is of interest since the organism can synthesize pentose from acetate, but cannot use hexoses for growth (1). In the course of the investigation into the enzymes associated with this synthesis, glyceraldehyde phosphate dehydrogenase activity, with either triphosphopyri...

The mechanism of triosephosphate dehydrogenase inactivation by reduced diphosphopyridine nucleotide.

We have shown in a previous communication (1) that the enzyme triosephosphate dehydrogenase is rapidly inactivated in the presence of reduced diphosphopyridine nucleotide at physiological concentrations, temperature, and pH. Good stoichiometry was shown for the amount of enzyme inactivation, DPNH-X1 formation, and disappearance of sulfhydryl groups. It was then of particular interest to determi...